| 1. | Relationship between platelet membrane gp b a and ischemic cardiocerebrovascular diseases
活性与缺血性心脑血管疾病 |
| 2. | Platelet active factor and platelet membrane glycoprotein associated with ischemic cerebrovascular disease
血小板活化因子及血小板膜糖蛋白与缺血性脑血管疾病 |
| 3. | During pregnancy mothers may become sensitized to platelet membrane antigens present on fetal platelets
在怀孕期内母亲也许会对胎儿的血小板细胞膜上表达的抗体过敏。 |
| 4. | Effects of ding xin recipe and tanshinose iia on expression of platelet membrane adhesive molecule in the rat of arrhythmia
对心律失常大鼠血小板膜粘附分子表达的影响 |
| 5. | Effects of yiqi huoxue tablet on expression of platelet membrane adhesion molecules in rats with cerebral infarction
益气活血片对脑梗塞大鼠血小板膜粘附分子表达的影响 |
| 6. | Diagnosis value of detecting platelet associated antibody and platelet membrane glycoprotein for immune thrombocytopenia
血小板相关抗体及膜糖蛋白检测在免疫性血小板减少症患者诊断中的价值 |
| 7. | The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase , to make the scfv - linker - uk32 chimeric gene . this gene was cloned into the transfer vector pbacpak9 , and cotransfected with bacpak6 bsu36i digest into sf 9 cells . the fusion protein was secreted into the medium . in the fifth day after the cotransfection , the supernatant of the medium showed 107 iu ml fibrinolytic activity , higher than 25 iu ml fibrinolytic activity of scfv - uk32 . elisa showed that the supernatant had the binding activity to activated platelet . wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
为了提高重组导向溶栓分子scfv - uk32的溶纤活性，通过重组pcr方法在编码scfv与uk32的碱基之间引入编码klgggg连接肽的碱基序列，并克隆到转移载体pbacpak9上,通过与线性病毒dna bacpak6 bsu36i digest共转染到昆虫细胞sf 9内，进行表达。表达产物分泌到上清中，共转染后第5d天用纤维平板法测得sf 9细胞上清溶纤活性达到107 iu ml ，比未引入连接肽的scfv - uk32的表达活性25 iu ml高。 |
